Regulatory
Part:BBa_K2510019:Design
Designed by: Alma Chapet--Batlle Group: iGEM17_Paris_Bettencourt (2017-10-27)
T7 - O-HKcI - O-TetR promoter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The promoter was designed based on the work of Zang et al. using their library of insulated operators
Source
This part was designed by the team and synthesized.
References
Zong, Y., Zhang, H., Lyu, C., Ji, X., Hou, J., Guo, X., Ouyang, Q. and Lou, C. (2017). Insulated transcriptional elements enable precise design of genetic circuits. Nature Communications, 8(1).